Spinal Fluid Protein Revisited: A Reappraisal of the Biuret Procedure
نویسندگان
چکیده
منابع مشابه
Assay of cerebrospinal fluid protein: a rate biuret method evaluated.
We evaluated a rate colorimetric method (Beckman) for measuring total protein in cerebrospinal fluid. The automated instrument we used was Beckman's ASTRA TM. A 100-microL sample of spinal fluid is introduced into the biuret reagent in the reaction cell and the increase in absorbance at 545 nm is monitored for 20.5 s. Solid-state circuits determine the rate of alkaline biuret-protein chelate fo...
متن کاملAssessment of a biuret method without concentration step for total protein determination in cerebrospinal fluid.
E xamination of cerebrospinal fluid (CSF) total protein is used chiefly to detect increased permeabili ty of the blood-brain barrier to plasma proteins or increased intrathecal secretion of immunoglobulins (1). A rapid and quite precise method is required. The biuret method is considered as a potential reference method for total protein determination in biological fluids (2-4). It has been modi...
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Vancomycin has been used for decades to treat serious systemic gram positive infections. Extensive use over time has demonstrated vancomycin is not nephrotoxic even when used in high dosage, i.e., twice the usual dose. Since vancomycin is not nephrotoxic, there is no rationale for dosing vancomycin based on serum vancomycin levels. Since vancomycin is eliminated by GFR, vancomycin dosing should...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1985
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1985.23.4.225